Gas chromatographic - mass spectrometric methods for the detection and identification of anabolic steroid drugs.

*Permission to carry out these drug studies was granted by the Northwick Park Hospital Ethical Committee. steroids. The columns were washed with water and the steroids recovered by elution with ethanol. The ethanol was taken to dryness and the residue dissolved in 0.5M acetate buffer (pH 4.6) and the steroid conjugates were hydrolysed by two enzymes present in snail digestive juice (Helix pomatia) and j3-glucuronidase. After 48 hours the free steroids were extracted on XAD-2 columns and eluted with ethanol.